Separation and identification of carp pituitary proteins and glycoproteins

Get it

Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α₁β and α₂β forms), growth hormone, free α₁ subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO₄-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)₂(Man)₃, were found. … full description

Bibliographic Details

Other Authors

Helena Ryšlavá, M. Janatová, G. Čalounová, Irena Selicharová, roz. Hulová, Jana Barthová, 1941- , Tomislav Barth, 1938-

Document Type

Articles

Physical Description

8 il.

Published in

Czech Journal of Animal Science. -- ISSN 1212-1819. -- Roč. 50, č. 9 (2005), s. 430-437

Subjects

FISHES

CARP

CYPRINUS CARPIO

ENDOCRINE GLANDS

PITUITARY GLAND

PITUITARY HORMONES

PROTEINS

GLYCOPROTEINS

CHROMATOGRAPHY

OLIGOSACCHARIDES

LH

SOMATOTROPIN

THYROTROPIN

ryby

kapr

Cyprinus carpio

endokrinní žlázy

hypofýza

hormony hypofýzy

bílkoviny

glykoproteiny

chromatografie

oligosacharidy

somatotropin

tyreotropin

Item Description

2 fot., 4 grafy, 2 tabulky

Bibliography

Bibliografie na s. 436-437 (27 zázn.)