Separation and identification of carp pituitary proteins and glycoproteins
Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α₁β and α₂β forms), growth hormone, free α₁ subunit and β subunit of thyroid-stimulating hormone … celý popis
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- Typ dokumentu
- Články
- Fyzický popis
- 8 il.
- Publikováno v
- Czech Journal of Animal Science. -- ISSN 1212-1819. -- Roč. 50, č. 9 (2005), s. 430-437
- Témata
- Popis jednotky
- 2 fot., 4 grafy, 2 tabulky
- Bibliografie
- Bibliografie na s. 436-437 (27 zázn.)
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MARC
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| 242 | 1 | 0 | |a Separace a identifikace bílkovin a glykoproteinů kapří hypofýzy |
| 245 | 0 | 0 | |a Separation and identification of carp pituitary proteins and glycoproteins / |c H. Ryšlavá ... [et al.] |
| 300 | |b 8 il. | ||
| 500 | |a 2 fot., 4 grafy, 2 tabulky | ||
| 504 | |a Bibliografie na s. 436-437 (27 zázn.) | ||
| 520 | 3 | |a Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α₁β and α₂β forms), growth hormone, free α₁ subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO₄-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)₂(Man)₃, were found. |9 eng | |
| 546 | |a Anglický text, anglický abstrakt | ||
| 650 | 0 | 9 | |a FISHES |2 agrovoc |
| 650 | 0 | 9 | |a CARP |2 agrovoc |
| 650 | 0 | 9 | |a CYPRINUS CARPIO |2 agrovoc |
| 650 | 0 | 9 | |a ENDOCRINE GLANDS |2 agrovoc |
| 650 | 0 | 9 | |a PITUITARY GLAND |2 agrovoc |
| 650 | 0 | 9 | |a PITUITARY HORMONES |2 agrovoc |
| 650 | 0 | 9 | |a PROTEINS |2 agrovoc |
| 650 | 0 | 9 | |a GLYCOPROTEINS |2 agrovoc |
| 650 | 0 | 9 | |a CHROMATOGRAPHY |2 agrovoc |
| 650 | 0 | 9 | |a OLIGOSACCHARIDES |2 agrovoc |
| 650 | 0 | 9 | |a LH |2 agrovoc |
| 650 | 0 | 9 | |a SOMATOTROPIN |2 agrovoc |
| 650 | 0 | 9 | |a THYROTROPIN |2 agrovoc |
| 650 | 0 | 7 | |a ryby |2 agrovoc |
| 650 | 0 | 7 | |a kapr |2 agrovoc |
| 650 | 0 | 7 | |a Cyprinus carpio |2 agrovoc |
| 650 | 0 | 7 | |a endokrinní žlázy |2 agrovoc |
| 650 | 0 | 7 | |a hypofýza |2 agrovoc |
| 650 | 0 | 7 | |a hormony hypofýzy |2 agrovoc |
| 650 | 0 | 7 | |a bílkoviny |2 agrovoc |
| 650 | 0 | 7 | |a glykoproteiny |2 agrovoc |
| 650 | 0 | 7 | |a chromatografie |2 agrovoc |
| 650 | 0 | 7 | |a oligosacharidy |2 agrovoc |
| 650 | 0 | 7 | |a LH |2 agrovoc |
| 650 | 0 | 7 | |a somatotropin |2 agrovoc |
| 650 | 0 | 7 | |a tyreotropin |2 agrovoc |
| 700 | 1 | |a Ryšlavá, Helena, |u Univerzita Karlova, Přírodovědecká fakulta, katedra biochemie, Praha |4 aut | |
| 700 | 1 | |a Janatová, M., |u Univerzita Karlova, Přírodovědecká fakulta, katedra biochemie, Praha |4 aut | |
| 700 | 1 | |a Čalounová, G., |u Univerzita Karlova, Přírodovědecká fakulta, katedra biochemie, Praha |4 aut | |
| 700 | 1 | |a Selicharová, Irena, |c roz. Hulová, |u Ústav organické chemie a biochemie AV ČR, Praha |4 aut | |
| 700 | 1 | |a Barthová, Jana, |d 1941- |7 jn20000710005 |u Univerzita Karlova, Přírodovědecká fakulta, katedra biochemie, Praha |4 aut | |
| 700 | 1 | |a Barth, Tomislav, |d 1938- |7 jn20000400073 |u Ústav organické chemie a biochemie AV ČR, Praha |4 aut | |
| 773 | 0 | |t Czech Journal of Animal Science |x 1212-1819 |g Roč. 50, č. 9 (2005), s. 430-437 |q 50:9 |9 2005 | |
| 856 | 4 | 1 | |u http://www.agriculturejournals.cz/publicFiles/53018.pdf |4 N |9 HTM |
| LKR | |a ITM |b 97022 |l UZP50 |y 2005 | ||
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| SIF | |z JA |d 20051228 | ||
| 910 | |a ABA009 |t rs | ||