Influence of chemotherapy on development and production of virus free in vitro strawberry plants

To chci

The objective of the study was to determine effects of ribavirin on development and health status of in vitro grown strawberry cultivars ‘Honeoye’ and ‘Elkat’ infected with viruses Strawberry mild yellow-edge virus (SMYEV), Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV). Antiviral compound ribavirin was added in concentrations 20, 40, 80 and 160 mg/l to the same MS medium as for multiplication. Growth reduction was noted on medium with 160 mg/l ribavirin and to a lesser degree in the 40 and 80 mg/l treatments. At the end of chemotherapy, in vitro clones free of viruses detected previously in the initial plants were obtained for both selected cultivars across all ribavirin concentrations. The highest number of plants (94) with negative results of ELISA testing was noted on medium with the highest ribavirin concentration 160 mg/l and the lowest (73) on medium with the lowest concentration 20 mg/l of ribavirin. The treated plants look symptomless and appear morphologically equal to the untreated control plants. Results indicate that ribavirin treatment of in vitro plants is a suitable method for eliminating SMYEV, ToRSV and ArMV from strawberry. … celý popis

Podrobná bibliografie

Hlavní autor

Jiří Sedlák

Další autoři

František Paprštein, 1949-
Jana Suchá

Typ dokumentu

Články

Fyzický popis

3 ilustrace

Publikováno v

Horticultural Science. -- ISSN 0862-867X. -- Roč. 46, č. 2 (2019), s. 53-56

Témata

berry crops

SOFT FRUITS

VARIETIES

EXPERIMENTAL INFECTION

Pathogenic viruses

NEPOVIRUSES

PLANT CONDITION

VIRUSFREE PLANTS

CULTURE MEDIA

IN VITRO CULTURE

VITROPLANTS

CLONES

SPECIFIC PATHOGEN FREE STATE

DNA FINGERPRINTING

ELISA

bobuloviny

Fragaria vesca

odrůdy

experimentální infekce

patogenní viry

nepoviry

zdravotní stav rostlin

bezvirózní rostliny

kultivační média

kultura in vitro

explantátové kultury

klony

symptomy

stav prostý specifických patogenů

identifikace DNA

virus okrajového žloutnutí listů jahodníku > ribavirin > antivirotika > virostatika > chemoterapie > eliminace virů

Popis jednotky

3 tabulky

Bibliografie

Seznam literatury na straně 56,